 | Shameik Moore | Miles Morales / Spider-Man (voice) |
 | Jake Johnson | Peter B. Parker / Spider-Man (voice) |
 | Hailee Steinfeld | Gwen Stacy / Spider-Woman (voice) |
 | Mahershala Ali | Uncle Aaron / Prowler (voice) |
 | Brian Tyree Henry | Jefferson Davis (voice) |
 | Lily Tomlin | Aunt May (voice) |
 | Lauren Vélez | Rio Morales (voice) |
 | Zoë Kravitz | Mary Jane (voice) |
 | John Mulaney | Peter Porker / Spider-Ham (voice) |
 | Kimiko Glenn | Peni Parker (voice) |
 | Nicolas Cage | Peter Parker / Spider-Man Noir (voice) |
 | Kathryn Hahn | Olivia 'Liv' Octavius / Doc Ock (voice) |
 | Liev Schreiber | Wilson Fisk / Kingpin (voice) |
 | Chris Pine | Peter Parker / Spider-Man (voice) |
 | Natalie Morales | Miss Calleros (voice) |
 | Edwin H. Bravo | Brooklyn Visions Security Guard (voice) |
 | Oscar Isaac | Interesting Person #1 (voice) |
 | Greta Lee | Interesting Person #2 (voice) |
 | Stan Lee | Stan (voice) |
 | Jorma Taccone | Green Goblin / Last Dude (voice) |
 | Joaquín Cosío | Scorpion (voice) |
 | Marvin 'Krondon' Jones III | Tombstone (voice) |
 | Kim Yarbrough | Scientist In Cafeteria (voice) |
 | Lake Bell | Vanessa Fisk (voice) |
 | Jessica Mikayla Adams | Brooklyn Friend (voice) |
| Gredel Berrios Calladine | Brooklyn Friend (voice) |
 | Sarah D. Cole | Brooklyn Friend (voice) |
| Kelby Joseph | Brooklyn Friend (voice) |
 | Mimi Davila | Brooklyn Visions Student (voice) |
 | Claudia Choi | Brooklyn Visions Teacher (voice) |
 | Melanie Haynes | Brooklyn Visions Teacher (voice) |
 | Joseph Izzo | Brooklyn Visions Teacher (voice) |
 | Nick Jaine | Brooklyn Visions Teacher (voice) |
 | Muneeb Rehman | Brooklyn Visions Teacher (voice) |
 | Carlos Zaragoza | Brooklyn Visions Teacher (voice) |
 | Post Malone | Brooklyn Bystander (voice) |
| David Applebee | Additional Voices (voice) |
 | Juan Carlos Arvelo | Additional Voices (voice) |
| Adam Brown | Additional Voices (voice) |
 | Jon Bruno | Additional Voices (voice) |
 | Darcy Rose Byrnes | Additional Voices (voice) |
 | Oscar Camacho | Additional Voices (voice) |
 | June Christopher | Additional Voices (voice) |
 | Alycia Cooper | Additional Voices (voice) |
 | Michelle Jubilee Gonzalez | Additional Voices (voice) |
 | Terrence Hardy Jr. | Additional Voices (voice) |
 | Bridget Hoffman | Additional Voices (voice) |
 | Rif Hutton | Additional Voices (voice) |
| Miguel Jiron | Additional Voices (voice) |
 | Harrison Knight | Additional Voices (voice) |
 | Lex Lang | Additional Voices (voice) |
 | Donna Lynn Leavy | Additional Voices (voice) |
| Andrew Leviton | Additional Voices (voice) |
 | Caitlin McKenna-Wilkinson | Additional Voices (voice) |
 | Scott Menville | Additional Voices (voice) |
 | Christopher Miller | Additional Voices (voice) |
| Arthur Ortiz | Additional Voices (voice) |
 | Juan Pacheco | Additional Voices (voice) |
 | Devika Parikh | Additional Voices (voice) |
 | Shakira Ja'nai Paye | Additional Voices (voice) |
 | Courtney Peldon | Additional Voices (voice) |
 | Chrystee Pharris | Additional Voices (voice) |
 | Jacqueline Pinol | Additional Voices (voice) |
 | Juan Pope | Additional Voices (voice) |
 | Al Rodrigo | Additional Voices (voice) |
 | Joseph Sanfelippo | Additional Voices (voice) |
 | Justin Shenkarow | Additional Voices (voice) |
 | Dennis Singletary | Additional Voices (voice) |
 | Warren Sroka | Additional Voices (voice) |
| Melissa Sturm | Additional Voices (voice) |
 | Holly Walker | Additional Voices (voice) |
 | Jason Linere-White | Additional Voices (voice) |
| Ruby Zalduondo | Additional Voices (voice) |
 | Ruth Zalduondo | Additional Voices (voice) |
97% 
93%