 | Shameik Moore | Miles Morales / Spider-Man (voice) |
 | Hailee Steinfeld | Gwen Stacy / Spider-Woman (voice) |
 | Jason Schwartzman | Jonathan Ohnn / Spot (voice) |
 | Oscar Isaac | Miguel O'Hara / Spider-Man 2099 (voice) |
 | Brian Tyree Henry | Jeff Morales (voice) |
 | Luna Lauren Velez | Rio Morales (voice) |
 | Jake Johnson | Peter B. Parker / Spider-Man (voice) |
 | Issa Rae | Jessica Drew / Spider-Woman (voice) |
 | Karan Soni | Pavitr Prabhakar / Spider-Man India (voice) |
 | Shea Whigham | George Stacy (voice) |
 | Greta Lee | LYLA (voice) |
 | Amandla Stenberg | Margo Kess: Spider-Byte (voice) |
 | Jharrel Jerome | Miles G. Morales (voice) |
 | Andy Samberg | Ben Reilly / Scarlet Spider (voice) |
 | Jack Quaid | Peter Parker / The Lizard (voice) |
 | Rachel Dratch | Ms. Weber (voice) |
 | Ziggy Marley | Lenny (voice) |
 | Jorma Taccone | Adriano Tumino / '67 (voice) |
 | Daniel Kaluuya | Hobie Brown / Spider-Punk (voice) |
 | Mahershala Ali | Uncle Aaron (voice) |
 | J.K. Simmons | J. Jonah Jameson (voice) |
 | Donald Glover | Aaron Davis |
 | Elizabeth Perkins | May / Quippy Spider-Person (voice) |
 | Kathryn Hahn | Doc Ock (voice) |
 | Ayo Edebiri | Glory (voice) |
| Nicole Delaney | MJ (voice) |
| Antonina Lentini | Betty (voice) |
 | Atsuko Okatsuka | Yuri (voice) |
 | Peter Sohn | Ganke (voice) |
 | Melissa Sturm | Mary Jane (voice) |
| Lorraine Velez | Maria (voice) |
 | Nic Novicki | Lego Spider-Man (voice) |
 | Taran Killam | Web-Slinger (voice) |
 | Metro Boomin | Metro Spider-Man (voice) |
 | Josh Keaton | Spectacular Spider-Man (voice) |
 | Sofia Barclay | Malala Windsor: Spider-UK (voice) |
 | Danielle Perez | Charlotte Webber: Sun-Spider (voice) |
 | Yuri Lowenthal | Insomniac Spider-Man (voice) |
| Rita Rani Ahuja | Additional Voices (voice) |
 | Ismail Bashey | Additional Voices (voice) |
 | Oscar Camacho | Additional Voices (voice) |
| Freddy Ferrari | Additional Voices (voice) |
| Kerry Gutierrez | Additional Voices (voice) |
| Kamal Khan | Additional Voices (voice) |
| Angelo Sekou Kouyate | Additional Voices (voice) |
| Andrew Leviton | Additional Voices (voice) |
 | David Michie | Additional Voices (voice) |
| Sumit Naig | Additional Voices (voice) |
 | Juan Pacheco | Additional Voices (voice) |
 | Chrystee Pharris | Additional Voices (voice) |
 | Ben Pronsky | Additional Voices (voice) |
 | Al Rodrigo | Additional Voices (voice) |
 | Stan Sellers | Additional Voices (voice) |
 | Warren Sroka | Additional Voices (voice) |
| Jasper Johannes Andrews | Additional Voices (voice) |
| Gredel Berrios Calladine | Additional Voices (voice) |
| Natalia Castellanos | Additional Voices (voice) |
| Russell Tyre Francis | Additional Voices (voice) |
 | Deepti Gupta | Additional Voices (voice) |
 | Sohm Kapila | Additional Voices (voice) |
| Pradnya Kuwadekar | Additional Voices (voice) |
| Ashley London | Additional Voices (voice) |
 | Christopher Miller | Additional Voices (voice) |
 | Andrea Navedo | Additional Voices (voice) |
| Lakshmi Patel | Additional Voices (voice) |
 | Jacqueline Pinol | Additional Voices (voice) |
 | Eliyas Qureshi | Additional Voices (voice) |
| Lashana Rodriguez | Additional Voices (voice) |
 | Jaswant Dev Shrestha | Additional Voices (voice) |
| Libby Thomas Dickey | Additional Voices (voice) |
 | Jason Linere-White | Additional Voices (voice) |
 | Sitara Attaie | Additional Voices (voice) |
| Mayuri Bhandari | Additional Voices (voice) |
 | June Christopher | Additional Voices (voice) |
 | Michelle Jubilee Gonzalez | Additional Voices (voice) |
 | Marabina Jaimes | Additional Voices (voice) |
 | Rez Kempton | Additional Voices (voice) |
 | Lex Lang | Additional Voices (voice) |
 | Phil Lord | Additional Voices (voice) |
| Richard Miro | Additional Voices (voice) |
| Doug Nicholas | Additional Voices (voice) |
 | Shakira Ja'nai Paye | Additional Voices (voice) |
| James Pirri | Additional Voices (voice) |
| Marley Ralph | Additional Voices (voice) |
 | Michelle Ruff | Additional Voices (voice) |
 | Dennis Singletary | Additional Voices (voice) |
 | Amanda Troop | Additional Voices (voice) |
 | Ruth Zalduondo | Additional Voices (voice) |
 | Kimberly Bailey | Additional Voices (voice) |
 | Sanjay Chandani | Additional Voices (voice) |
| Melanie Duke | Additional Voices (voice) |
 | Jorge R. Gutierrez | Additional Voices (voice) |
 | Miguel Jiron | Additional Voices (voice) |
| Deepti Kingra-Mickelsen | Additional Voices (voice) |
 | Luisa Leschin | Additional Voices (voice) |
 | Caitlin McKenna | Additional Voices (voice) |
| Andrew Morgado | Additional Voices (voice) |
 | Arthur Ortiz | Additional Voices (voice) |
| Eliana A. Perez | Additional Voices (voice) |
 | Juan Pope | Additional Voices (voice) |
 | Mike Rianda | Additional Voices (voice) |
| Erika Scopelli | Additional Voices (voice) |
| Narender Sood | Additional Voices (voice) |
 | Cedric L. Williams | Additional Voices (voice) |
 | Kimiko Glenn | Peni Parker (voice) (uncredited) |
 | Peggy Lu | Mrs. Chen (archive footage) (uncredited) |
 | Andrew Garfield | Peter Parker / Spider-Man (archive footage) (uncredited) |
 | Denis Leary | Captain Stacy (archive footage) (uncredited) |
 | Tobey Maguire | Peter Parker / Spider-Man (archive footage) (uncredited) |
 | Cliff Robertson | Ben Parker (archive footage) (uncredited) |
 | Alfred Molina | Doctor Octopus (voice) (archive footage) (uncredited) |
 | Post Malone | Brooklyn Bystander (voice) (archive footage) (uncredited) |
95% 
94%